Colorimetric determination of mucin dispersions and its application for bioadhesive evaluation of pegylated nanoparticles

Authors: J. M. Irache, K. Yoncheva,

Summary. The aim of the present study was to evaluate the possibility for quantitative colorimetric determination of mucin by using Micro BCA reagent. Series of mucin dispersions (concentration range 100-500 .g/ml) were prepared in distilled water, acidic and phosphate buffers. The results obtained in the three media showed good linearity and a possibility for quantitative mucin determination by colorimetry (measurement at 570 nm). Further, the capacity of pegylated poly(anhydride) nanoparticles to interact with mucin was evaluated applying the quantitative determination of mucin fractions adsorbed on the nanoparticles. Different amounts of mucin were adsorbed on the various types of the nanoparticles (modified with PEG and mPEG), which was attributed to the different surface properties of the particles.

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