High pressure liquid chromatographic method for simultaneous determination of ketoprofen and omeprazole

Authors: B. Tsvetkova, L. Peikova, S. Bozhanov, V. Maslarska,

Abstract

A simple, specific, precise and accurate reversed phase liquid chromatographic method has been proposed for the simultaneous determination of ketoprofen and omeprazole in laboratorymixture. The chromatographic separation was performed on a LiChrosorb C18, 125 mm x 4.6 mm, 5 μmcolumn at a detector wavelength of 230 nm and a flow rate of 1.5 ml/min. The mobile phase was composed of phosphate buffer (pH adjudted to 4.6 with ortho-phosphoric acid) and methanol (25:75v/v)

The retention times of ketoprofen and omeprazole were found to be 3.05 and 6.96 min, respectively. The method was validated for the parameters like specificity, linearity, precision, accuracy, limit of quantitation and limit of detection. The calibration curves were linear in the concentration range of 25.00-200.0 μg/ml for ketoprofen and 5.00-40.00 μg/ml for omeprazole. The estimated relative standard deviation values of % recovery at accuracy studies were found to be not greater than 3 %.

The presented method for the simultaneous determination of ketoprofen and omeprazole in synthetic mixture is specific, rapid and simple with good sensitivity.

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