Abstract. A simple, sensitive and specifi c liquid chromatographic method with UV detection was developed for the simultaneous estimation of Amlodipine Besylate (AML) and Valsartan (VAL) in tablet dosage form. Separation was achieved with a C18 (250 mm x 4.6 mm, 10 μm) column, ambient temperature with isocratic mode with mobile phase containing acetonitrile and 0.5 M potassium dihydrogen phosphate buffer pH 2.5 adjusted with ortho-phosphoric acid (35:65). The fl ow rate was 1.5 ml/min and eluent was monitored at 226nm. The selected chromatographic conditions were found to effectively to separate Amlodipine Besylate and Valsartan with retention time of 5.49 min and 10.19 min., respectively. The calibration curves were linear in the concentration range of 5.00-40.00 μg/ml for AML and 80.00-640.0 μg/ml for VAL. Intra- and interday relative standard deviations for both the components were <2.0 %. The proposed method was found to be accurate, precise, reproducible and specifi c and it can also be used for routine quality control analysis of the drugs in combination tablets.