RP-HPLC method for simultaneous determination of Ibuprofen and Famotidine in pharmaceutical dosage form

personАвтори: B. Tsvetkova, L. Peikova, M. Georgieva


In this study, reversed phase high performance liquid chromatographic method have been developed and validated for the simultaneous determination of ibuprofen and famotidine in combined pharmaceutical formulation. Separation was achieved with a C8 (250 mm x 4.6 mm, 5 μm) column, ambient temperature with isocratic mode with mobile phase containing acetonitrile and 0.5 M potassium dihydrogen phosphate buffer pH 2.2 adjusted with ortho-phosphoric acid (25:75). UV detection was performed at 280 nm. The flow rate was 1.2 ml/min. The retention times of ibuprofen and famotidine were found to be 3.19 min and 8.37 min, respectively. The responses were linear (R2 > 0.9999) in therange of 20 – 160 μg/ml for ibuprofen and 0.68 – 5.4μg/ml for famotidine. The % recovery for ibuprofen and famotidinewas 99.75 and 99.24, respectively. No chromatographic interference from the tablet excipients was found. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which can be applied for the routine analysis of ibuprofen and famotidine in tablet dosage forms

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