Abstract.

A 2,4-dimethyl-1H-chromeno[4′,3′:4,5]pyrido[2,3-d]pyrimidine-1,3,7(2H,4H)-trione 3 was synthesized via a one-pot procedure. Itsstructure was established on the basis of FTIR, ¹H NMR, ¹³C NMR,DEPT, COSY, HSQC, MS data and elemental analysis. The new compound was assayed for antiproliferative/cytotoxic effects in a panel of human tumor cell lines after 72 h exposure. MTT-dye reduction assay was used as a read-out system and IC₅₀ as an end-point to evaluate efficacy. Our cytotoxicity screening indicates that the presented fused system exhibits moderate antiproliferative effect in a panel of human tumor cell lines of different origin and cellular type.