RP-HPLC method for determination of naproxen in pharmaceutical dosage form

personАвтори: B. Tsvetkova

Abstract. A simple, sensitive and specific liquid chromatographic method with UV detection was developed for the determination of Naproxen in tablet dosage form. Separation was achieved with a C18 (250 mm x 4.6 mm, 10 μm) column, ambient temperature with isocratic mode with mobile phase containing acetonitrile:0.5 M potassium dihydrogen phosphate buffer pH 2.5 adjusted with ortho-phosphoric acid:tetrahydrofuran (45:53:2 v/v/v). The flow rate was 1.0 ml/min and eluent was monitored at 254 nm. The selected chromatographic conditions were found to effectively separate naproxen with retention time of 3.25 min. The calibration curve was linear in the concentration range of 12.50-100.00μg/ml. The proposed method was found to be accurate, precise, reproducible and specific and it can also be used for routine quality control of the analysed drug in tablets.

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