Abstract
In this study, reversed phase high performance liquid chromatographic method have been developed and validated for the determination of ciprofloxacin in tablets. Separation was achieved with a C18 (250 mm x 4.6 mm, 5 μm) column, temperature of 33ºC with isocratic mode with mobile phase containing 0.3% orto-phosphoric acid and acetonitril (65:35). UV detection was performed at 290 nm. The flow rate was 0.8 ml/min. The retention time of ciprofloxacin was found to be 3.10 min. The response was linear (R2 0.9999) in therange of 12.5 – 100 μg/ml. The % recovery was estimated as 98.96±2.14. No chromatographic interference from the tablet excipients was found. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which can be applied for the routine analysis of ciprofloxacin in tablet dosage forms.