Abstract:

Application of itaconic acid at the dose of 20 mg/cm² on the intact skin of white rats within 20 days causes elimination of anaerobic bacteria of Eubacterium species and partial elimination of autochthonous obligate anaerobic bacteria of Bifidobacterium species as well as facultative anaerobic and aerobic bacteria of Enterococcus species from the near-epithelial biological film of the colon mucosa. Incontrast the near-epithelial biofilm of the colon mucosa is contaminated with enterobacteria (bacteria of Proteus, Edwardsiella, Klebsiella species), and bacteria of Clostridium, Staphylococcus species.

The use of the above-mentioned dose of itaconic acid within 20 days leads to profound disorders of the colonizational resistance of colon mucosa as a result of marked deficiency of autochthonous obligate anaerobic bacteria of Bifidobacterium, Lactobacillus, Bacteroides, Enbacteriumspecies and facultative anaerobic and aerobic bacteria of Enterococcus, Escherichia species.

Bacteria of Clostridium species, enterobacteria of Proteus, Edwardsiella, Klebsiella species and opportunistic bacteria of Staphylococcus species, that contaminate the near-epithelial biofilm of colon mucosa in white rats, reach moderate (from 3.66±0.17 to 4.89± 0.15 LgCFU/g) population level, coefficient of quantitative dominance (from 29.1 to 57.3) and  index of relevance (from 0.02 to 0.09).