Using three-dimensional fluorescent spectroscopy while studying drugs on the basis of apiculture products.

personАвтори: A. М. Kotenko, L. I. Tscheblykina, Yu. V. Chernykh, А. I. Tikhonov, О. S. Shpichak


The three-dimensional fluorescence spectra of the drugs on the basis of apiculture products have been studied, the conformity of the spectra with the chemical composition of the drugs studied has been shown. Two intensive peaks of fluorescence in the range of λem – 320 nm (λexc – 260 nm) and λem – 345 nm (λexc – 240 nm) characteristic for the unsaturated fatty acids have been seen on 3-DF-spectrum of the bee pollen lipophilic extract. 3-DF- spectrum of the complex drug with the extract of the big bee moth biomass has the fluorescence peak in the range of λem – 315-330 nm (λexc – 270 nm), which is characteristic for the unsaturated lipids, as well as a number of peaks in the range of λem – 650-750 nm (λexc – 400-430, 505, 540, 610, 650-680 nm) characteristic for the mixture of chlorophylls А and В. While studying 3-DF-spectra of the individual components of suppositories with hydrophobic propolis drug and Hippophaё oil as well as their mixtures, it has been found that the points of the spectrum maxima of the suppositories correspond to the points of the spectrum maxima of the individual components; it testifies indirectly the absence of the chemical interaction between the components. The 3-DF-spectra of apidrugs obtained have the individual character and it gives the possibility of using them for identification.

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